Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Evaluation of PFS with high or low abundance of CTCs by using meta-analysis ( a - c ) and systematic review ( d ). a Higher abundance of CTCs predicts shorter PFS of NSCLC. b Stratified study found that the differences of heterogeneity among tumor stages are quite small(I 2 = 0%). c Stratified study found that the differences of heterogeneity among various detect methods of CTCs are moderate(I 2 = 36.1%). d Systematic review of PFS (months) data indicated higher abundance of CTCs are asscociated with shorter PFS

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques:

Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Evaluation of OS with high or low abundance of CTCs by using meta-analysis ( a - c ) and systematic review ( d ). a . Higher abundance of CTCs had not significant predict power of prognosis of lung cancer cases. b Stratified analysis showed the differences of tumor stages in different cohorts lead to heterogeneity(I 2 = 81.5%). c Stratified analysis showed various detect methods in different cohorts lead to heterogeneity (I 2 = 79.0%). d Systematic review of OS (months) data indicated higher abundance of CTCs are associated with shorter OS

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques:

Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Summary of the published studies included in the meta-analysis and system analysis

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques: Biomarker Discovery

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Multiple-target aptamer-based microfluidics for the detection of circulating tumor cells (CTCs). (A) Combining multiplex surface-enhanced Raman scattering (SERS) nanovectors and multivariate analysis for in situ profiling of CTC phenotypes using a microfluidic chip. (B) Simultaneous single-cell phenotype analysis of hepatocellular carcinoma CTCs using a SERS-aptamer-based microfluidic chip. (C) A poly(lactic- co -glycolic acid) (PLGA) nanofiber microfluidic device for highly efficient isolation and release of CTCs with different phenotypes based on dual aptamers. (D) Dual-multivalent-aptamer-conjugated nanoprobes for superefficient discerning of single CTCs in a microfluidic chip with inductively coupled plasma-mass spectrometry (ICP-MS) detection. EGFR: epidermal growth factor receptor; EpCAM: epithelial cell adhesion molecule; HER2: human epidermal growth factor receptor-2; HCC: hepatocellular carcinoma; WBC: white blood cells; RBC: red blood cells; MGITC: malachite green isothiocyanate; DTDC: 3,3′-diethylthiadicarbocyanine iodide; AuNPs: gold nanoparticles; PDMS: polydimethylsiloxane; PLGA: poly(lactic- co -glycolic acid); F i : inertial lift forces; F d : dean drag force; DMA: dual-multivalent-aptamers. Reprinted from Refs. [ , , , ] with permission.

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: Multiplex Assay, In Situ, Isolation, Clinical Proteomics, Mass Spectrometry

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Aptamer-based microfluidic for the detection of circulating tumor cells (CTCs) and extracellular vesicles (EVs).

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: Fluorescence, Modification, Isolation, In Situ

Journal: Journal of Pharmaceutical Analysis

Article Title: Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

doi: 10.1016/j.jpha.2023.03.001

Figure Lengend Snippet: Multiple-target aptamer-based microfluidics for fluorescent detection of extracellular vesicles (EVs). (A) A microfluidic coflow system using two labelled aptamers with Cy5-conjugated human epidermal growth factor receptor-2 (HER2) aptamer and fluorescein amidite (FAM)-conjugated epithelial cell adhesion molecule (EpCAM) aptamer to visualize the separation process. (B) A thermophoretic aptasensor using a panel of seven fluorescent Cy5-conjugated aptamers to label serum EVs. MVB: multivesicular body; EXO: exosomes; MVs: microvesicles; ABs: apoptotic bodies; TBE: tris-boric acid-ethylenediamine tetraacetic acid; F e : the centerline-directed elastic lift force; F i : inertial lift force; F d : viscous drag force; a: diameter of EV; LDA: linear discriminant analysis; sCMOS: scientific complementary metal-oxide semiconductor. Reprinted from Refs. [ , ] with permission.

Article Snippet: SYL3C , EpCAM , MCF-7 , CTCs and single-target simple aptamer-based microfluidics , Aptamer-modified magnetic nanoclusters; biomimetic microfluidic device; and fluorescence analysis , [ ] .

Techniques: